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Detection of Pathogens and Ampicillin-resistance Genes Using Multiplex Padlock Probes —BIO-PROTOCOL

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Abstract designation assays for infective agent identification and characterization are limited either by the performance of simultaneously perceptible targets, which rely on multiplexing methods, or by time constraints due to cultivation-based techniques. We recently conferred a 100-plex method for human pathogen characterization to regard 75 microorganism and plant species as well as 33 clinically at issue β-lactamases (Barišić , 2016). By using 16S r RNA cistron sequences as barcode ingredient in the padlock probes, and two assorted fluorescence channels for species and antibiotic resistance identification, we managed to cut the number of microarray probes needed by half.

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A coumarin-based fluorescent probe for selective and sensitive detection of thiophenols and its application. | Anal Chem;86(6): 3037-42, 2014 Mar 18. | MEDLINE | Portal Regional da BVS

The development of probes for particularised thiophenol detection is of great importance, due to the toxicity of thiophenols and their derivatives in the environment. In the present study, a novel fluorescent probe was rationally designed for detecting thiophenols via an intramolecular charge transfer mechanism. The developed probe by selection and sensitively distinguished thiophenols from aliphatic thiols.

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